these activities may need to be further explored and exploited in the pursuit of newer contraceptives.Globally, road traffic accidents are a major cause of death and severe injuries. It is estimated that the number of deaths on the world's roads at 1.5 million per annum puts road traffic injuries as the eighth leading cause of death globally. Understanding the influence of environmental factors on deaths and severe injuries will help in policy-making and the development of strategies in Limpopo Province. We, therefore, aim to study environmental factors that influence road deaths and severe injuries and to identify whether their impact on injury severity levels varies. The study was based on secondary data on road traffic accidents obtained from the Department of Roads and Transport in Limpopo Province. The data comprised 18 029 road traffic accidents for the period January 2009-December 2015. The study found that weekends (Saturdays and Sundays) had the highest number of accidents when compared to weekdays. The proportion of observations in each severity level was not constant across explanatory variables. The generalized ordered logit regression (GOLR) models seemed to be an effective predicting model that can be adapted to determine the influence of environmental factors on injury severity compared to the ordered logit regression (OLR) model. The results of the GOLR model suggest that environmental factors such as slippery road conditions, rainy weather, and spring season lower the likelihood of severe crash occurrence. On the other hand, poor or defective road surface, time interval (6 a.m. to 11 p.m.), and provincial roads have a higher likelihood of severe crash occurrence. To decrease the severity of injuries in the province, provincial roadways must be maintained regularly.Revascularization of the pulp tissue is one of the fundamental processes and challenges in regenerative endodontic procedures (REPs). In this regard, the current study is aimed at synthesizing the mineral trioxide aggregate- (MTA-) based scaffolds as a biomaterial for REPs. learn more Poly (ε-caprolactone) (PCL)/chitosan (CS)/MTA scaffolds were constructed and evaluated by FTIR, SEM, XRD, and TGA analyses. Proliferation and adhesion of human dental pulp stem cells (hDPSCs) were assessed on these scaffolds by scanning electron microscopy (SEM) and MTT assays, respectively. The expression of angiogenic markers was investigated in gene and protein levels by real-time PCR and western blotting tests. Our results indicated that the obtained appropriate physicochemical characteristics of scaffolds could be suitable for REPs. The adhesion and proliferation level of hDPSCs were significantly increased after seeding on PCL/CS/MTA scaffolds. The expression levels of VEGFR-2, Tie2, and Angiopoietin-1 genes were statistically increased on the PCL/CS/MTA scaffold. In support of these findings, western blotting results showed the upregulation of these markers at protein levels in PCL/CS/MTA scaffold (P less then 0.05). The current study results suggested that PCL/CS/MTA scaffolds provide appropriate structures for the adhesion and proliferation of hDPSCs besides induction of the angiogenesis process in these cells.
The main purpose of this study was to assess the radiopacity of contemporary restorative computer-aided design (CAD)/computer-aided manufacturing (CAM) materials and the impact of material composition as measured by energy-dispersive X-ray spectrophotometry (EDX) on radiopacity.

Ten specimens of six CAD/CAM materials with 1 mm thickness were produced and then digitally radiographed with an aluminum (Al) step-wedge (SW) and 1 mm thick tooth slice. The specimen mean gray values (MGVs) were recorded in pixels and compared to an Al-SW, dentin, and enamel of equal thickness. For the elementary analysis of the composition of the materials, EDX was performed.

The recorded MGVs ranged between 21.20 ± 4.94 and 238.5 ± 13.61 pixels. Materials were sorted according to the MGVs descendingly, Prettau, Vita Suprinity, Vita Enamic, Shofu, Pekkton, and BioHPP. Prettau and Vita Suprinity had significantly higher MGV than dentin and 1 mm thick Al. In comparison, Vita Enamic had a slightly higher value than dentin and 1 mm thick Al. Although Pekkton and BioHPP had MGV significantly lower than dentin and 1 mm thick Al, Shofu had a significantly lower value than dentin and nonsignificantly lower than 1 mm thick Al (
 < 0.05). According to EDX analysis, the examined materials contained several components in varying quantities of radiopacity.

The radiopacity of only three studied materials exceeded the International Organization for Standardization's minimum standards (ISO).
The radiopacity of only three studied materials exceeded the International Organization for Standardization's minimum standards (ISO).
The obesity epidemic is considered one of the main challenges for modern medicine. It has been proven that bariatric surgery is more effective than nonsurgical interventions to manage weight-related comorbidities. The general surgeon needs to understand how tooth erosion advances in gastroesophageal reflux in people who have had bariatric surgery. Dental erosion caused due to gastric reflux begins with the enamel, which is the tough, protective coating that covers our teeth. While it is tough, it is prone to an extremely acidic environment with a low pH, where it begins to soften and demineralize, gradually wearing away and exposing the more sensitive areas of the tooth. Because of the growing popularity of this subspecialty, general surgeons should develop a basic clinical and surgical understanding of these standard procedures and complications, regardless of their interest in obesity surgery.

This cross-sectional study aimed to assess the awareness and attitude of surgeons regarding dental erosion on pations.
The general surgeons had inadequate awareness and attitude regarding dental erosion on bariatric surgery patients. Our findings suggest that a lack of adequate awareness and a negative attitude among general surgeons are grounds for concern and that more should be done to avoid oral health complications.This study evaluated the physical and mechanical properties of glass ionomer cement (GIC) associated with 5% hydroxyapatite nanoparticles (NPHAps) and 10% bioactive glass (BAG) 45S5 before and after brushing at different storage times. Surface roughness was evaluated using a rugosimeter, Vickers hardness using a microdurometer, and mass variation measured in an analytical balance at 1, 7, 15, 30, and 60 days before and after the brushing test, with the aid of toothbrushing simulator and soft bristle toothbrushes. Nonnormal distribution was observed, and the nonparametric Wilcoxon and Kruskal-Wallis tests followed by Dunn's were performed, with a significance level of 5%. We observed higher values for mass loss on the first day for all groups. The surface roughness was lower in the control and NP groups, 30 days after brushing. Higher values for hardness were found in the control group and lower ones for NP, after brushing. The control and BAG groups presented a decrease in hardness over time. The NP group presented the highest values before brushing, while the control group had the highest values after brushing. The association of NPHPa with the GIC is the most promising combination, since it presented satisfactory values for surface hardness. However, conventional GIC not associated with NPHPa or BAG is still an option, since it is available in the market and the most economically viable option.The SARS-CoV-2 pandemic from 2019 onwards has significantly increased the usage of surgical style medical masks, both in healthcare and public settings. It is important to study the contamination of and viral transfer from such masks. However, accepted standard test methods such as ISO 18184 have prescribed inoculation methods which may not be fully representative of the type of viral insult experienced in the clinic or community. In addition to studying a conventional mask, the performance of a mask featuring an antimicrobial photosensitiser was also studied.Acute lymphoblastic leukemia (ALL) is the most common type of childhood cancer, with a feature of easy to induce multidrug resistance and relapse. Abundant studies have proved that iron overload strengthens the growth and metastasis of tumor cells. Herein, we found that deferoxamine (DFO) effectively decreased the concentration of intracellular iron in ALL cells. DFO inhibited proliferation, induced apoptosis, and obstructed cell cycle of ALL cells, whereas DFO and dextriferron (Dex) used in combination significantly decreased the sensitivity of ALL cells to DFO. Reactive oxygen species (ROS) level was reduced in ALL cells treated with DFO, and the combination of DFO and Dex reversed the effects of DFO. In vivo, DFO inhibited mouse tumor growth. Besides, cyclinD1, β-catenin, c-Myc, hypoxia inducible factor 1 (HIF-1), p-p38MAPK, and p-ERK1/2 protein levels were significantly downregulated, and the levels of prolyl hydroxylase-2 (PHD-2) were upregulated after treated with DFO, whereas Dex treatment reversed those in vivo and in vitro. In conclusion, DFO inhibited the proliferation and ALL xenograft tumor growth, obstructed the cell cycle, and induced apoptosis of ALL cells, probably via inactivating the ROS/HIF-1α, Wnt/β-catenin, and p38MAPK/ERK signaling.
Aberrant expression of tripartite motif 11 (TRIM11) and the Wnt/
-catenin pathway are essential for facilitating tumorigenesis and progression in multiple types of cancer.

To investigate the molecular changes linking the dysregulation of TRIM11 and Wnt/
-catenin pathway activation in gastric cancer (GC) progression.

The expression levels of TRIM11 were detected in GC tissues and cells by immunohistochemistry and western blotting. The role of TRIM11 in the growth, proliferation, and invasion of gastric cancer cells was observed by a series of cell functional experiments and further verified in vivo. Co-immunoprecipitation (Co-IP), immunofluorescence, cycloheximide, and western blotting assays and other experiments were conducted to explore the mechanisms of TRIM11 underlying the regulation of the Wnt/
-catenin pathway. For further verification, rescue experiments were performed by cotransfection of TRIM11 and Axin1 siRNA in GC cells.

Using Co-IP assays, we identified TRIM11 as a potent binding partne therapeutic target for the treatment of GC patients.
This study aimed to review the potential chemoprotective effects of curcumin against the doxorubicin-induced cardiotoxicity.

According to the PRISMA guideline, a comprehensive systematic search was performed in different electronic databases (Web of Science, PubMed, and Scopus) up to July 2021. One hundred and sixty-four studies were screened in accordance with a predefined set of inclusion and exclusion criteria. Eighteen eligible articles were finally included in the current systematic review.

According to the in vitro and in vivo findings, it was found that doxorubicin administration leads to decreased cell survival, increased mortality, decreased bodyweight, heart weight, and heart to the bodyweight ratio compared to the control groups. However, curcumin cotreatment demonstrated an opposite pattern in comparison with the doxorubicin-treated groups alone. Other findings showed that doxorubicin significantly induces biochemical changes in the cardiac cells/tissue. Furthermore, the histological changes on the cardiac tissue were observed following doxorubicin treatment.learn more